INTRACELLULAR α-AMYLASE IN BACILLUS SUBTILIS
نویسندگان
چکیده
منابع مشابه
Bottleneck in secretion of α-amylase in Bacillus subtilis
Amylase plays an important role in biotechnology industries, and Gram-positive bacterium Bacillus subtilis is a major host to produce heterogeneous α-amylases. However, the secretion stress limits the high yield of α-amylase in B. subtilis although huge efforts have been made to address this secretion bottleneck. In this question-oriented review, every effort is made to answer the following que...
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BACKGROUND Alkaline α-amylases have potential applications for hydrolyzing starch under high pH conditions in the starch and textile industries and as ingredients in detergents for automatic dishwashers and laundries. While the alkaline α-amylase gains increased industrial interest, the yield of alkaline α-amylases from wild-type microbes is low, and the combination of genetic engineering and p...
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The hyperthermophilic α-amylase from Thermococcus sp. HJ21 possesses unique traits (Ca(2+)-independent thermostability and optimal temperature of 95°C) that make it a great potential candidate for use in the food industry. However, this Archaea isolated from a deep-sea thermal vent requires strict control of culture conditions and produces only small amounts of α-amylase. To solve these problem...
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The aim of this study was to isolate and express the randomly mutated α-amylase gene from B. subtilis strain 168. BS168F: 5'-gtgtcaagaatgtttgc-3' and BS168R: 3'-gttttgttaaaagatga-5' primers were used to amplify the amylase gene using the following cycle in error-prone PCR method: 94°C for 30 s, 40°C for 2 min, and 72°C for 2 min in 30 cycles that were followed with 72°C for 2 min as a post cycl...
متن کاملThe Theoretical Study on a Nano Biosystem Consisting of Nano Tube-Catalytic Site of Bacillus Subtilis α-Amylase, PDB: 1UA7
α-Amylase has been studied extensively from various sides. This enzyme is used in many industries .Many applications of this enzyme have encouraged us for greater attempts on the study of α-amylase and to search for more effective processes. In this investigation, the structure of nanotube - catalytic site of bacillus subtilis α- amylase was optimized by hype...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1963
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.85.1.246-247.1963